A549 Whole Cell Lysate
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application Notes | Ready-to-use A549 whole cell lysate is prepared as a positive control for separation by SDS-PAGE and subsequent western blot analysis. Heat lysate to 95°C for 5 minutes and rapidly cool. The recommended loading volume per lane is 2~10 ul depending on the size format of your gel. |
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Format | Whole lysate is stored in 1 X SDS 0.3% beta-mercaptoethanol sample buffer. |
Storage | Store vial at -70°C or colder. For extended storage, aliquot contents to minimize freeze/thaw cycles. Storage at -20°C will be stable for three months, storage at-70°C will be stable for one year. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
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