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YWHAG Antibody (N-term) [Knockout Validated]
Affinity Purified Rabbit Polyclonal Antibody (Pab) KO
![WB - YWHAG Antibody (N-term) [Knockout Validated] AW5704-U100](/assets/uploads/products/201311/AP2943a_WB_1.jpg)
![WB - YWHAG Antibody (N-term) [Knockout Validated] AW5704-U100](/assets/uploads/products/200912/AW5704_WB_1.jpg)
![IHC-P - YWHAG Antibody (N-term) [Knockout Validated] AW5704-U100](/assets/uploads/products/200912/AP2943a_21180_ihc_1.jpg)
![FC - YWHAG Antibody (N-term) [Knockout Validated] AW5704-U100](/assets/uploads/products/200912/AP2943a_21180_fc_1.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC |
|---|---|
| Primary Accession | P61981 |
| Other Accession | Q6NRY9, Q6PCG0, Q6PC29, P61983, P61982, Q5F3W6, P68252 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Zebrafish, Chicken, Bovine, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 28303 Da |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 7532 |
|---|---|
| Antigen Region | 63-92 aa |
| Other Names | 14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma, N-terminally processed, YWHAG |
| Dilution | WB~~1:500-1:2000 IHC-P~~1:10~50 FC~~1:10~50 |
| Target/Specificity | This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human YWHAG. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | YWHAG Antibody (N-term) [Knockout Validated] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | YWHAG |
|---|---|
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed:16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed:36732624). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P68252}. |
| Tissue Location | Highly expressed in brain, skeletal muscle, and heart. |
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Provided below are standard protocols that you may find useful for product applications.
Background
YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
References
Jagemann,L.R., et.al., J. Biol. Chem. 283 (25), 17450-17462 (2008)
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