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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ANKH Antibody (C-term)   

ANKH Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ANKH Antibody (C-term) AP9741B
    Anti-ANKH Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ANKH Antibody (C-term) AP9741B
    Western blot analysis of ANKH Antibody (C-term) (Cat. #AP9741b) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ANKH Antibody (C-term) AP9741B
    ANKH Antibody (C-term) (Cat. #AP9741b) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ANKH Antibody (C-term) AP9741B
    ANKH Antibody (C-term) (Cat. #AP9741b) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9HCJ1
Other Accession P58366, Q9JHZ2
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54241 Da
Antigen Region 464-492 aa
Additional Information
Gene ID 56172
Other Names Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581
Target/Specificity This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANKH Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANKH {ECO:0000303|PubMed:35147247, ECO:0000312|HGNC:HGNC:15492}
Function Transports adenosine triphosphate (ATP) and possibly other nucleoside triphosphates (NTPs) from cytosol to the extracellular space. Mainly regulates their levels locally in peripheral tissues while playing a minor systemic role. Prevents abnormal ectopic mineralization of the joints by regulating the extracellular levels of the calcification inhibitor inorganic pyrophosphate (PPi), which originates from the conversion of extracellular NTPs to NMPs and PPis by ENPP1 (PubMed:32639996, PubMed:35147247, PubMed:20943778). Regulates the release of the TCA cycle intermediates to the extracellular space, in particular citrate, succinate and malate. Extracellular citrate mostly present in bone tissue is required for osteogenic differentiation of mesenchymal stem cells, stabilization of hydroxyapatite structure and overall bone strength (PubMed:32639996). The transport mechanism remains to be elucidated (Probable).
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells
Research Areas
Citations (0)
citation

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Background

ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.

References

Wang, J., et al. J. Rheumatol. 36(6):1265-1272(2009)
Ho, A.M., et al. Science 289(5477):265-270(2000)
Rojas, K., et al. Genomics 62(2):177-183(1999)

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$ 365.00
$ 140.00
Cat# AP9741B
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