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EFEMP1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EFEMP1 Antibody (N-term) AP9095a
    Western blot analysis of EFEMP1 Antibody (N-term) (Cat. #AP9095a) in mouse lung tissue lysates (35ug/lane). EFEMP1 (arrow) was detected using the purified Pab.
    detail
  • WB - EFEMP1 Antibody (N-term) AP9095a
    All lanes : Anti-EFEMP1 Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - EFEMP1 Antibody (N-term) AP9095a
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EFEMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - EFEMP1 Antibody (N-term) AP9095a
    EFEMP1 Antibody (N-term) (Cat. #AP9095a) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 5
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q12805
Other Accession O35568, Q8BPB5, Q7YQD7
Reactivity Human, Mouse
Predicted Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54641 Da
Antigen Region 119-148 aa
Additional Information
Gene ID 2202
Other Names EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3, EFEMP1, FBLN3, FBNL
Target/Specificity This EFEMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the N-terminal region of human EFEMP1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEFEMP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EFEMP1
Synonyms FBLN3, FBNL
Function Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
Cellular Location Secreted, extracellular space. Secreted, extracellular space, extracellular matrix Note=Localizes to the lamina propria underneath the olfactory epithelium.
Tissue Location In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina
Research Areas
Citations ( 0 )

Background

EFEMP1 encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors.

References

Okada,Y., et.al., Hum. Mol. Genet. (2010) In press
Wakabayashi,T., et.al., Biochem. Biophys. Res. Commun. 391 (1), 1116-1121 (2010)

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$ 365.00
$ 140.00
Cat# AP9095a
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