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PCM-1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q15154 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 228560 Da |
| Antigen Region | 632-661 aa |
| Gene ID | 5108 |
|---|---|
| Other Names | Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1 |
| Target/Specificity | This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 632-661 amino acids from the Central region of human PCM-1. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PCM-1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PCM1 (HGNC:8727) |
|---|---|
| Function | Required for centrosome assembly and function (PubMed:12403812, PubMed:15659651, PubMed:16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed:12403812, PubMed:15659651). Required to anchor microtubules to the centrosome (PubMed:12403812, PubMed:15659651). Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed:20551181, PubMed:24121310, PubMed:27979967). Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed:34782749). |
| Cellular Location | Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8AV28}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body Note=Recruitment to the centrosome requires microtubules and dynein The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase Displaced from centriolar satellites and centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling |
| Tissue Location | Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid. |
Provided below are standard protocols that you may find useful for product applications.
Background
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.
References
Balczon R., Bao L.J. Cell Biol. 124:783-793(1994)
Corvi R., Berger N.Oncogene 19:4236-4242(2000)
Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002)
Reiter A., Walz C.Cancer Res. 65:2662-2667(2005)
Murati A., Gelsi-Boyer V.Leukemia 19:1692-1696(2005)
Hames R.S., Crookes R.E.Mol. Biol. Cell 16:1711-1724(2005)
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