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CBS Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CBS Antibody (Center) AP6959c
    All lanes : Anti-CBS Antibody (Center) at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: LNCap whole cell lysate Lane 4: mouse liver lysate Lane 5: mouse pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IF - CBS Antibody (Center) AP6959c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling CBS with AP6959c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
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  • WB - CBS Antibody (Center) AP6959c
    All lanes : Anti-CBS Antibody (Center) at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: LNCaP whole cell lysate Lane 5: Human liver lysate Lane 6: Mouse liver lysate Lane 7: Mouse pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - CBS Antibody (Center) AP6959c
    Immunohistochemical analysis of AP6959C on paraffin-embedded Human liver tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
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  • IHC-P-Leica - CBS Antibody (Center) AP6959c
    Immunohistochemical analysis of AP6959C on paraffin-embedded Human brain tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
    detail
  • FC - CBS Antibody (Center) AP6959c
    Overlay histogram showing Hela cells stained with AP6959c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6959c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P-Leica, FC, IF, E
Primary Accession P35520
Other Accession Q58H57, P0DN79
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 60587 Da
Antigen Region 301-330 aa
Additional Information
Gene ID 102724560;875
Other Names Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS
Target/Specificity This CBS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from the Central region of human CBS.
Dilution WB~~1:2000
IF~~1:25
IHC-P-Leica~~1:500
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBS
Function Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L- homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed:23981774, PubMed:20506325, PubMed:23974653). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).
Cellular Location Cytoplasm. Nucleus
Tissue Location In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney
Research Areas
Citations ( 0 )

Background

CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.

References

Ravel,C., et.al., PLoS ONE 4 (8), E6540 (2009)

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$ 365.00
$ 140.00
Cat# AP6959c
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