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STX7 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - STX7 Antibody (N-term) AP5670A
    Western blot analysis of STX7 Antibody (N-term) (Cat. #AP5670a) in MDA-MB231 cell line lysates (35ug/lane).STX7 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - STX7 Antibody (N-term) AP5670A
    STX7 Antibody (N-term) (Cat. #AP5670a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STX7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - STX7 Antibody (N-term) AP5670A
    STX7 Antibody (N-term) (Cat. #AP5670a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O15400
Other Accession O70257, O70439, Q3ZBT5, NP_003560.2
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29816 Da
Antigen Region 47-73 aa
Additional Information
Gene ID 8417
Other Names Syntaxin-7, STX7
Target/Specificity This STX7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-73 amino acids from the N-terminal region of human STX7.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTX7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STX7
Function May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
Cellular Location Early endosome membrane; Single- pass type IV membrane protein
Tissue Location Highest expression is found in placenta followed by heart, skeletal muscle, kidney and brain. Low expression is found in pancreas, lung and liver
Research Areas
Citations (0)
citation

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Background

Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

References

Prekeris, R., et al. Mol. Biol. Cell 10(11):3891-3908(1999)
Steegmaier, M., et al. J. Biol. Chem. 273(51):34171-34179(1998)
Wong, S.H., et al. J. Biol. Chem. 273(1):375-380(1998)
Wang, H., et al. Gene 199 (1-2), 39-48 (1997)

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$ 182.50
$ 70.00
Cat# AP5670A
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