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Phospho-cJun(S63) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Phospho-cJun(S63) Antibody AP3072a
    Western blot analysis of anti-Phospho-cJun-pS63 Pab (Cat. #AP3072a) in mouse kidney tissue lysate (35ug/lane). cJun-pS63 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - Phospho-cJun(S63) Antibody AP3072a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
  • DB - Phospho-cJun(S63) Antibody AP3072a
    Dot blot analysis of anti-Phospho-cJun-S63 Antibody (Cat.#AP3072a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, DB, E
Primary Accession P05412
Other Accession P17325, P05627, O77627
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35676 Da
Additional Information
Gene ID 3725
Other Names Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Target/Specificity This cJun Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.
Dilution WB~~1:1000
IHC-P~~1:50~100
DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-cJun(S63) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name JUN
Function Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Cellular Location Nucleus.
Tissue Location Expressed in the developing and adult prostate and prostate cancer cells.
Research Areas
Citations (0)
citation

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Background

This gene for the cJun protein is the putative transforming gene of avian sarcoma virus 17. The protein is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene for this protein is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

References

Fang, D., et al., Proc. Natl. Acad. Sci. U.S.A. 101(41):14782-14787 (2004).
Wang, Y., et al., Biochem. Biophys. Res. Commun. 323(1):9-16 (2004).
Wehkamp, J., et al., Infect. Immun. 72(10):5750-5758 (2004).
Gensch, E., et al., J. Biol. Chem. 279(37):39085-39093 (2004).
Fujioka, S., et al., Mol. Cell. Biol. 24(17):7806-7819 (2004).

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$ 365.00
$ 140.00
Cat# AP3072a
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