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GSTO1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GSTO1 Antibody (Center) AP2930C
    Western blot analysis of GSTO1 Antibody (Center) (Cat. #AP2930c) in NCI-H460 cell line lysates (35ug/lane). GSTO1 (arrow) was detected using the purified Pab.
    detail
  • WB - GSTO1 Antibody (Center) AP2930C
    Western blot analysis of GSTO1 (arrow) using rabbit polyclonal GSTO1 Antibody (Center) (Cat. #AP2930c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTO1 gene.
    detail
  • WB - GSTO1 Antibody (Center) AP2930C
    Anti-GSTO1 Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - GSTO1 Antibody (Center) AP2930C
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with GSTO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GSTO1 Antibody (Center) AP2930C
    GSTO1 Antibody (Center) (Cat. #AP2930c) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P78417
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27566 Da
Antigen Region 126-155 aa
Additional Information
Gene ID 9446
Other Names Glutathione S-transferase omega-1, GSTO-1, Glutathione S-transferase omega 1-1, GSTO 1-1, Glutathione-dependent dehydroascorbate reductase, Monomethylarsonic acid reductase, MMA(V) reductase, S-(Phenacyl)glutathione reductase, SPG-R, GSTO1, GSTTLP28
Target/Specificity This GSTO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human GSTO1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTO1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTO1
Synonyms GSTTLP28
Function Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
Cellular Location Cytoplasm, cytosol.
Tissue Location Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart Lowest expression in brain, placenta and lung
Research Areas
Citations (0)
citation

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Background

GSTO1 is a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, this protein acts as a small stress response protein, likely involved in cellular redox homeostasis.

References

Wang,Y.H., et.al., Toxicol. Appl. Pharmacol. 241 (1), 111-118 (2009)

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$ 182.50
$ 70.00
Cat# AP2930C
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