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DLK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DLK1 Antibody (C-term) AP20959c
    All lanes : Anti-DLK1 Antibody (C-term) at 1:1000 dilution Lane 1: human placenta lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - DLK1 Antibody (C-term) AP20959c
    Immunohistochemical analysis of paraffin-embedded H. placenta section using DLK1 Antibody (C-term)(Cat#AP20959c). AP20959c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • FC - DLK1 Antibody (C-term) AP20959c
    Flow cytometric analysis of HepG2 cells using DLK1 Antibody (C-term)(green, Cat#AP20959c) compared to an isotype control of mouse IgG2b(blue). AP20959c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P80370
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41300 Da
Antigen Region 368-402 aa
Additional Information
Gene ID 8788
Other Names Protein delta homolog 1, DLK-1, pG2, Fetal antigen 1, FA1, DLK1, DLK
Target/Specificity This DLK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 368-402 amino acids from the C-terminal region of human DLK1.
Dilution WB~~1:1000
IHC-P~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDLK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DLK1
Synonyms DLK
Function May have a role in neuroendocrine differentiation.
Cellular Location Membrane; Single-pass type I membrane protein. Cytoplasm {ECO:0000250|UniProtKB:O70534}
Tissue Location Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue
Research Areas
Citations ( 0 )

Background

May have a role in neuroendocrine differentiation.

References

Laborda J.,et al.J. Biol. Chem. 268:3817-3820(1993).
Lee Y.L.,et al.Biochim. Biophys. Acta 1261:223-232(1995).
Helman L.J.,et al.Nucleic Acids Res. 18:685-685(1990).
Heilig R.,et al.Nature 421:601-607(2003).
Jensen C.H.,et al.Eur. J. Biochem. 225:83-92(1994).

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$ 365.00
$ 140.00
Cat# AP20959c
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