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SSNA1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SSNA1 Antibody (N-term) AP20483a
    SSNA1 Antibody (N-term) (Cat. #AP20483a) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SSNA1 antibody detected the SSNA1 protein (arrow).
    detail
  • IHC-P - SSNA1 Antibody (N-term) AP20483a
    Immunohistochemical analysis of paraffin-embedded H. kideny section using SSNA1 Antibody (N-term)(Cat#AP20483A). AP20483A was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • IHC-P - SSNA1 Antibody (N-term) AP20483a
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SSNA1 Antibody (N-term)(Cat#AP20483A). AP20483A was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession O43805
Other Accession Q9JJ94, Q5E9C3
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 13596 Da
Antigen Region 25-53 aa
Additional Information
Gene ID 8636
Other Names Sjoegren syndrome nuclear autoantigen 1, Nuclear autoantigen of 14 kDa, SSNA1, NA14
Target/Specificity This SSNA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 amino acids from the N-terminal region of human SSNA1.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSSNA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SSNA1 (HGNC:11321)
Function Microtubule-binding protein which stabilizes dynamic microtubules by slowing growth and shrinkage at both plus and minus ends and serves as a sensor of microtubule damage, protecting microtubules from the microtubule-severing enzyme SPAST (PubMed:34970964). Induces microtubule branching which is mediated by the formation of long SSNA1 fibrils which guide microtubule protofilaments to split apart from the mother microtubule and form daughter microtubules (By similarity). Plays a role in axon outgrowth and branching (PubMed:25390646). Required for cell division (PubMed:25390646).
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Midbody. Cytoplasm, cytoskeleton, flagellum basal body. Cytoplasm, cytoskeleton, flagellum axoneme. Cell projection, axon {ECO:0000250|UniProtKB:Q9JJ94}. Note=In sperm, strongly expressed in the basal body region with weaker expression in the axoneme (PubMed:12640030). Localizes to axon branching points in neurons (By similarity). {ECO:0000250|UniProtKB:Q9JJ94, ECO:0000269|PubMed:12640030}
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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References

Ramos-Morales F., et al. J. Biol. Chem. 273:1634-1639(1998).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Halleck A., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., et al. Nature 429:369-374(2004).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP20483a
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