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DVL3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - DVL3 Antibody (C-term) AP12030b
    Confocal immunofluorescent analysis of DVL3 Antibody (C-term)(Cat#AP12030b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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  • WB - DVL3 Antibody (C-term) AP12030b
    All lanes : Anti-DVL3 Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: K562 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - DVL3 Antibody (C-term) AP12030b
    DVL3 Antibody (C-term) (Cat. #AP12030b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DVL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession Q92997
Other Accession Q61062, NP_004414.3
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 78055 Da
Antigen Region 530-557 aa
Additional Information
Gene ID 1857
Other Names Segment polarity protein dishevelled homolog DVL-3, Dishevelled-3, DSH homolog 3, DVL3, KIAA0208
Target/Specificity This DVL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-557 amino acids from the C-terminal region of human DVL3.
Dilution IF~~1:10~50
WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDVL3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DVL3
Synonyms KIAA0208
Function Involved in the signal transduction pathway mediated by multiple Wnt genes.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O14641}.
Research Areas
Citations (0)
citation

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Background

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation.

References

Gnad, T., et al. Mol. Cancer 9, 31 (2010) :
Bouteille, N., et al. Oncogene 28(28):2569-2580(2009)
Fukukawa, C., et al. Oncogene 28(8):1110-1120(2009)
Wei, Q., et al. Lung Cancer 62(2):181-192(2008)
Lee, Y.N., et al. Cell. Signal. 20(2):443-452(2008)

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$ 182.50
$ 70.00
Cat# AP12030b
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