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LMO4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - LMO4 Antibody (Center) AP10757C
    Fluorescent image of A549 cell stained with LMO4 Antibody (Center)(Cat#AP10757c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).LMO4 immunoreactivity is localized to Nucleus significantly.
    detail
  • WB - LMO4 Antibody (Center) AP10757C
    LMO4 Antibody (Center) (Cat. #AP10757c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the LMO4 antibody detected the LMO4 protein (arrow).
    detail
  • FC - LMO4 Antibody (Center) AP10757C
    LMO4 Antibody (Center) (Cat. #AP10757c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession P61968
Other Accession P61969, Q3SWZ8, NP_006760.1
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 17994 Da
Antigen Region 104-131 aa
Additional Information
Gene ID 8543
Other Names LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4, LMO-4, LMO4
Target/Specificity This LMO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human LMO4.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLMO4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LMO4
Function Transcription cofactor. Plays a role in establishing motor neuron identity, in concert with MNX1, acting, at least in part, to disrupt LDB1-LHX3 complexes thereby negatively modulating interneuron genes in motor neurons.
Research Areas
Citations (0)
citation

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Background

This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene.

References

Tian, Y., et al. Clin. Exp. Metastasis 27(7):455-463(2010)
Ribases, M., et al. Biol. Psychiatry 66(10):926-934(2009)
Montanez-Wiscovich, M.E., et al. Oncogene 28(41):3608-3618(2009)
Huang, Z., et al. Dev. Biol. 327(1):132-142(2009)
Caporaso, N., et al. PLoS ONE 4 (2), E4653 (2009) :

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$ 365.00
$ 140.00
Cat# AP10757C
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